Oxygen-pulse curves in rat liver mitochondrial suspensions. Some observations and deductions
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چکیده
منابع مشابه
Oxygen-pulse curves in rat liver mitochondrial suspensions. Some observations and deductions.
1. The inference, implicit in the chemiosmotic hypothesis, that protons move into the bulk phase during ATP synthesis was investigated. 2. Incubation of rat liver mitochondria in the presence of the cation exchanger CM-Sephadex C-50 caused alkalinization in the medium, though total ATP synthesis remained unchanged. The addition of N-ethylmaleimide prevented the alkalinization, but there was sti...
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Suspensions of single cells were prepared from normal adult rat liver by perfusion of liver with and shaking in buffered solutions of 0.1% hyaluronidase and 0.05 % collagenase. L-[lJ4C]Leucine and L-[lJ4C]methionine incorporated into protein could be measured by precipitating the cells on filter paper disks without prior homogenization. No incorporation of amino acids into protein was observed ...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1979
ISSN: 0264-6021
DOI: 10.1042/bj1800161